Haynes Lab:Notebook/CRISPR Editing/2015/08/17: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
|-
| 23||P159||P176
|-
|-
| 29||P149||P174
| 29||P149||P174
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|-
|-
| 33||P149||P176
| 33||P149||P176
|-
| 35||P149||P174
|-
|-
| 36||P149||P174
| 36||P149||P174
|-
| 38||P165||P160
|-
|-
| 40||P163||P160
| 40||P163||P160
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|-
|-
| 43||P213||P214
| 43||P213||P214
|-
| 44||P213||P214
|-
| 45||P163||P160
|-
| 49||P213||P214
|}
|}


<br><br>
Found some triplicate samples of Luc14 and Gal4-EED treated samples for g023, g044, g045. PCR'd with P197/198. Checked on a gel, negative controls blank. Made 1:1000 dilutions and ran nested PCRd with the following primers:


{| {{table}}
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
|-
|-
| 23||P159||P176
| 23||P149||P215
|-
| ||||
|-
| 30||P159||P215
|-
| ||||
|-
| 29||P149||P174
|-
| 35||P149||P174
|-
| 36||P149||P174
|-
| ||||
|-
| 33||P149||P176
|-
| ||||
|-
| 38||P165||P160
|-
| ||||
|-
| 41||P175||P214
|-
| ||||
|-
| 42||P213||P214
|-
| 43||P213||P214
|-
|-
| 44||P213||P214
| 44||P213||P214
|-
|-
| 49||P213||P214
| 45||P151||P160
|-
| ||||
|-
| 40||P163||P160
|-
| 45||P163||P160
|}
|}






gRNA F primer R primer
23 P159 P176
30 P159 P215
29 P149 P174
35 P149 P174
36 P149 P174
33 P149 P176
38 P165 P160
41 P175 P214
42 P213 P214
43 P213 P214
44 P213 P214
49 P213 P214
40 P163 P160
45 P163 P160
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 12:37, 19 August 2015

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08/17/2015

Phusion PCR for singlet full coverage of all gRNAs tested

gRNA F primer R primer
29 P149 P174
30 P159 P215
33 P149 P176
36 P149 P174
40 P163 P160
41 P175 P214
42 P213 P214
43 P213 P214



Found some triplicate samples of Luc14 and Gal4-EED treated samples for g023, g044, g045. PCR'd with P197/198. Checked on a gel, negative controls blank. Made 1:1000 dilutions and ran nested PCRd with the following primers:

gRNA F primer R primer
23 P149 P215
44 P213 P214
45 P151 P160



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