Haynes Lab:Notebook/CRISPR Editing/2015/08/25
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08/25/2015Transfect gal4+puro and gal4+dox with g034, collect at 24, 48, and 72 hours Step 1: Making .5ug/10ul plasmid stocks
Step 2: make DNA + optimem mixes
Step 3: add plus reagent to DNA+ opti
step 4: Make optimem + lipo mastermix
Step 5: mix dna mix and lipo mix
Step 6: add lipo mix to cells
Transfected 2 wells for each siRNA and each cell type
For blank transfections
Mix optimem and siRNA or water
Mix oligofectamine and optimem
Mix DNA and oligofectamine
While complexes form, prep cells
Add complexes to cells
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