Haynes Lab:Notebook/CRISPR Editing/2015/09/02: Difference between revisions

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Rerunning surveyor on g048 gal4 puro/dox samples from 7/20/15. First time I ran them there was a lot of background which I attributed to the old Surveyor kit. Now I have a new kit and will see if there is still background. If so, will redo the nested PCR with another set of primers.  
Rerunning surveyor on g048 gal4 puro/dox siRNA samples from 7/20/15. First time I ran them there was a lot of background which I attributed to the old Surveyor kit. Now I have a new kit and will see if there is still background. If so, will redo the nested PCR with another set of primers.  




Latest revision as of 01:06, 27 September 2017

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09/02/2015

Running surveyor on singlet Luc14 rep 1 g023, g044, and g045 and the test PCRs from yesterday, Luc14 g034 1.0ug plasmid rep 1, P149/176, two templates, gDNA and P197/198 PCR product.



Rerunning surveyor on g048 gal4 puro/dox siRNA samples from 7/20/15. First time I ran them there was a lot of background which I attributed to the old Surveyor kit. Now I have a new kit and will see if there is still background. If so, will redo the nested PCR with another set of primers.



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