Haynes Lab:Notebook/CRISPR Editing/2015/09/05: Difference between revisions

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(Autocreate 2015/09/05 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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| colspan="2"|
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==03//2015==
==09/05/2015==
 
purified PCR products for luc14 g034 dilution experiment. anneal for surveyor
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''plasmid dil'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Luc14||g034||1ug||1||0.093||0.048||1.914||92.658||4.32||1.50||9.18
|-
| Luc14||g034||1ug||2||0.106||0.056||1.898||106.083||3.77||1.50||9.73
|-
| Luc14||g034||1ug||3||0.071||0.035||1.997||70.777||5.65||1.50||7.85
|-
| Luc14||g034||0.1ug||1||0.093||0.049||1.871||92.535||4.32||1.50||9.18
|-
| Luc14||g034||0.1ug||2||0.078||0.041||1.9||78.231||5.11||1.50||8.39
|-
| Luc14||g034||0.1ug||3||0.069||0.037||1.845||69.116||5.79||1.50||7.71
|-
| Luc14||g034||0.01ug||1||0.086||0.046||1.882||86.235||4.64||1.50||8.86
|-
| Luc14||g034||0.01ug||2||0.094||0.05||1.89||94.463||4.23||1.50||9.27
|-
| Luc14||g034||0.01ug||3||0.078||0.043||1.801||77.628||5.15||1.50||8.35
|}




Revision as of 11:33, 5 September 2015

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09/05/2015

purified PCR products for luc14 g034 dilution experiment. anneal for surveyor

Cell type gRNA plasmid dil rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Luc14 g034 1ug 1 0.093 0.048 1.914 92.658 4.32 1.50 9.18
Luc14 g034 1ug 2 0.106 0.056 1.898 106.083 3.77 1.50 9.73
Luc14 g034 1ug 3 0.071 0.035 1.997 70.777 5.65 1.50 7.85
Luc14 g034 0.1ug 1 0.093 0.049 1.871 92.535 4.32 1.50 9.18
Luc14 g034 0.1ug 2 0.078 0.041 1.9 78.231 5.11 1.50 8.39
Luc14 g034 0.1ug 3 0.069 0.037 1.845 69.116 5.79 1.50 7.71
Luc14 g034 0.01ug 1 0.086 0.046 1.882 86.235 4.64 1.50 8.86
Luc14 g034 0.01ug 2 0.094 0.05 1.89 94.463 4.23 1.50 9.27
Luc14 g034 0.01ug 3 0.078 0.043 1.801 77.628 5.15 1.50 8.35



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