Haynes Lab:Notebook/CRISPR Editing/2015/09/08: Difference between revisions

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(Autocreate 2015/09/08 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==09/08/2015==
 
Purified PCR products, set up annealing reactions
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''time post xfect'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4||dox||g034||24||1||0.076||0.042||1.813||76.239||5.25||1.50||8.25
|-
| Gal4||dox||g034||24||2||0.087||0.048||1.812||87.35||4.58||1.50||8.92
|-
| Gal4||dox||g034||24||3||0.08||0.043||1.851||80||5.00||1.50||8.50
|-
| Gal4||puro||g034||24||1||0.085||0.048||1.788||85.425||4.68||1.50||8.82
|-
| Gal4||puro||g034||24||2||0.05||0.029||1.725||50.174||7.97||1.50||5.53
|-
| Gal4||puro||g034||24||3||0.078||0.044||1.783||77.729||5.15||1.50||8.35
|}




Revision as of 08:18, 8 September 2015

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09/08/2015

Purified PCR products, set up annealing reactions

Cell type drug gRNA time post xfect rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4 dox g034 24 1 0.076 0.042 1.813 76.239 5.25 1.50 8.25
Gal4 dox g034 24 2 0.087 0.048 1.812 87.35 4.58 1.50 8.92
Gal4 dox g034 24 3 0.08 0.043 1.851 80 5.00 1.50 8.50
Gal4 puro g034 24 1 0.085 0.048 1.788 85.425 4.68 1.50 8.82
Gal4 puro g034 24 2 0.05 0.029 1.725 50.174 7.97 1.50 5.53
Gal4 puro g034 24 3 0.078 0.044 1.783 77.729 5.15 1.50 8.35



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