Haynes Lab:Notebook/CRISPR Editing/2015/09/10
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09/10/2015Ran g031 P197/198 PCR samples on gel. No band in water-only control PCR. Gal4-EED +dox g031 rep 2 failed. Re-ran with 5ul of gDNA and it worked. gDNA extraction of g025 and g044 treated cells
Set up phusion PCR with P197/198 with 200ng gDNA |