Haynes Lab:Notebook/CRISPR Editing/2015/10/06: Difference between revisions

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(Autocreate 2015/10/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==10/06/2015==
Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel


<br><br>
Col pur Gal4 puro/dox g054 samples, set up annealing reaction
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||puro||g054||1||0.1||0.054||1.87||100.189||3.99||1.50||9.51
|-
| Gal4-EED||puro||g054||2||0.09||0.047||1.91||90.466||4.42||1.50||9.08
|-
| Gal4-EED||puro||g054||3||0.105||0.057||1.862||105.5||3.79||1.50||9.71
|-
| Gal4-EED||dox||g054||1||0.104||0.054||1.919||103.727||3.86||1.50||9.64
|-
| Gal4-EED||dox||g054||2||0.115||0.063||1.825||114.985||3.48||1.50||10.02
|-
| Gal4-EED||dox||g054||3||0.092||0.049||1.891||92.038||4.35||1.50||9.15
|}
<br><br>
Col pur Gal4 puro/dox g055 samples, set up annealing reaction




Revision as of 08:07, 6 October 2015

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10/06/2015

Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel



Col pur Gal4 puro/dox g054 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g054 1 0.1 0.054 1.87 100.189 3.99 1.50 9.51
Gal4-EED puro g054 2 0.09 0.047 1.91 90.466 4.42 1.50 9.08
Gal4-EED puro g054 3 0.105 0.057 1.862 105.5 3.79 1.50 9.71
Gal4-EED dox g054 1 0.104 0.054 1.919 103.727 3.86 1.50 9.64
Gal4-EED dox g054 2 0.115 0.063 1.825 114.985 3.48 1.50 10.02
Gal4-EED dox g054 3 0.092 0.049 1.891 92.038 4.35 1.50 9.15



Col pur Gal4 puro/dox g055 samples, set up annealing reaction



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