Haynes Lab:Notebook/CRISPR Editing/2015/10/07: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2015/10/07 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==10/07/2015== | ||
PCR purification | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''drug''' | |||
| align="center" style="background:#f0f0f0;"|'''siRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''drug''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul ann buff''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g025||1||0.104||0.057||1.834||104.399||3.83||1.50||9.67 | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g025||2||0.087||0.046||1.876||86.528||4.62||1.50||8.88 | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g025||3||0.096||0.052||1.821||95.567||4.19||1.50||9.31 | |||
|- | |||
| Gal4-EED||dox||none||puro||g025||1||0.117||0.063||1.863||116.936||3.42||1.50||10.08 | |||
|- | |||
| Gal4-EED||dox||none||puro||g025||2||0.077||0.043||1.813||77.314||5.17||1.50||8.33 | |||
|- | |||
| Gal4-EED||dox||none||puro||g025||3||0.088||0.046||1.901||88.068||4.54||1.50||8.96 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''drug''' | |||
| align="center" style="background:#f0f0f0;"|'''siRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''drug''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul ann buff''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g034||1||0.054||0.031||1.755||54.126||7.39||1.50||6.11 | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g034||2||0.063||0.035||1.829||63.131||6.34||1.50||7.16 | |||
|- | |||
| Gal4-EED||dox||EZH2||puro||g034||3||0.054||0.032||1.706||54.267||7.37||1.50||6.13 | |||
|- | |||
| Gal4-EED||dox||none||puro||g034||1||0.068||0.038||1.81||68.014||5.88||1.50||7.62 | |||
|- | |||
| Gal4-EED||dox||none||puro||g034||2||0.052||0.029||1.811||52.032||7.69||1.50||5.81 | |||
|- | |||
| Gal4-EED||dox||none||puro||g034||3||0.05||0.028||1.777||50.273||7.96||1.50||5.54 | |||
|} | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| 0.132||0.071||1.864||132.19 | |||
|} | |||
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Revision as of 14:08, 7 October 2015
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10/07/2015PCR purification
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