Haynes Lab:Notebook/CRISPR Editing/2015/11/08: Difference between revisions
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==11/08/2015== | ==11/08/2015== | ||
Nested PCR of g032 Luc14 samples (last run had a band in the negative ctrl lane)<br>Ran on gel. | Nested PCR of g032 Luc14 samples (last run had a band in the negative ctrl lane)<br>Ran on gel. light band in neg ctrl lane. Purified anyway. | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Cell type''' | |||
| align="center" style="background:#f0f0f0;"|'''gRNA''' | |||
| align="center" style="background:#f0f0f0;"|'''rep''' | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul ann buff''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
|- | |||
| Luc14||g032||1||0.077||0.042||1.849||77.174||5.18||1.50||8.32 | |||
|- | |||
| Luc14||g032||2||0.083||0.044||1.898||83.264||4.80||1.50||8.70 | |||
|- | |||
| Luc14||g032||3||0.094||0.052||1.806||93.826||4.26||1.50||9.24 | |||
|} | |||
<br><br> | <br><br> |
Revision as of 08:34, 8 November 2015
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11/08/2015Nested PCR of g032 Luc14 samples (last run had a band in the negative ctrl lane)
Total of 9 with enzyme and 9 without
With enzyme MM
Without enzyme MM
Incubate at 37°C for 15 minutes, heat inactivate at 80°C for 5 minutes. |