Haynes Lab:Notebook/CRISPR Editing/2016/02/05: Difference between revisions

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#Luc14 2, cleaned and sonicated
#Luc14 2, cleaned and sonicated
#1kb+ ladder
#1kb+ ladder
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[[Image:Screenshot 2016-03-12 20.37.14.png]]
<br>
<br>
Spec'd on nanodrop
Spec'd on nanodrop

Revision as of 20:38, 12 March 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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02/05/2016

Cass purified and ran sonicated DNA on gel
Lanes

  1. 1kb+ ladder
  2. Gal4 EED 1, unsonicated
  3. Gal4 EED 1, uncleaned and sonicated
  4. Gal4 EED 1, cleaned and sonicated
  5. Gal4 EED 2, unsonicated
  6. Gal4 EED 2, uncleaned and sonicated
  7. Gal4 EED 2, cleaned and sonicated
  8. Luc14 1, unsonicated
  9. Luc14 1, uncleaned and sonicated
  10. Luc14 1, cleaned and sonicated
  11. Luc14 2, unsonicated
  12. Luc14 2, uncleaned and sonicated
  13. Luc14 2, cleaned and sonicated
  14. 1kb+ ladder



Spec'd on nanodrop

Gal4 EED 1, cleaned and sonicated: 152.9 ng/uL
Gal4 EED 2, cleaned and sonicated: 204.7 ng/uL
Luc14 1, cleaned and sonicated: 99.2 ng/uL
Luc14 2, cleaned and sonicated: 122.2 ng/uL


Put samples at -20deg


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