Haynes Lab:Notebook/CRISPR Editing/2016/02/22: Difference between revisions

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Line 22: Line 22:
# " Luc14 2
# " Luc14 2
# " dummy
# " dummy
Gel/ Buffer
* NuPAGE 4-12% BisTris




Revision as of 15:00, 22 February 2016

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03//2015

Gel lanes

  1. stock: ladder
  2. sample prep: Luc14 1 (14 uL protein)
  3. sample prep: Luc14 2 (14 uL protein)
  4. sample prep: dummy (14 uL water)
  5. " ladder
  6. " Luc14 1
  7. " Luc14 2
  8. " dummy
  9. " ladder
  10. " Luc14 1
  11. " Luc14 2
  12. " dummy

Gel/ Buffer

  • NuPAGE 4-12% BisTris


Sample prep

  • 5 ul 4x Loading Buffer, 1 uL 10M DTT, 14 uL protein or water


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