Haynes Lab:Notebook/CRISPR Editing/2016/02/22: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==03//2015==
==03//2015==
 
Followed this [https://benchling.com/hayneslab/f/8iVwOlNf-protein-and-enzyme-assays/prt-HlvWjOID-sds-page/edit | [protocol]]
Gel lanes
Gel lanes


Revision as of 17:45, 22 February 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

03//2015

Followed this | [protocol] Gel lanes

  1. stock: ladder
  2. sample prep: Luc14 1 (14 uL protein)
  3. sample prep: Luc14 2 (14 uL protein)
  4. sample prep: dummy (14 uL water)
  5. " ladder
  6. " Luc14 1
  7. " Luc14 2
  8. " dummy
  9. " ladder
  10. " Luc14 1
  11. " Luc14 2
  12. " dummy


Gel/ Buffer

  • NuPAGE 4-12% BisTris
  • NuPAGE® MOPS SDS Running Buffer


Sample prep

  • 5 ul 4x Loading Buffer, 1 uL 10M DTT, 14 uL protein or water


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2016/02/22&oldid=931060"