Haynes Lab:Notebook/CRISPR Editing/2016/02/22: Difference between revisions
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Transfer and blocking following this [https://benchling.com/hayneslab/f/8iVwOlNf-protein-and-enzyme-assays/prt-fiQyr0E7-western-blot/edit protocol].<br> | Transfer and blocking following this [https://benchling.com/hayneslab/f/8iVwOlNf-protein-and-enzyme-assays/prt-fiQyr0E7-western-blot/edit protocol].<br> | ||
Remove the gel from the box, rinse with DI water, scrape the exposed gel slit on the bottom to get all the gel from that spot. Rinse the gel gunk away. Crack the plastic apart but don't pull it apart until the plastic is unstuck. With the gel submerged in a tupperware filled with DI water, separate the plastic pieces. Pay attention to which plastic part the gel is sticking to. Pay attention to the direction of your gel. Get the gel floating in the DI water. <br> | Remove the gel from the box, rinse with DI water, scrape the exposed gel slit on the bottom to get all the gel from that spot. Rinse the gel gunk away. Crack the plastic apart but don't pull it apart until the plastic is unstuck. With the gel submerged in a tupperware filled with DI water, separate the plastic pieces. Pay attention to which plastic part the gel is sticking to. Pay attention to the direction of your gel. Get the gel floating in the DI water. <br> | ||
Transfer the gel to nitrocellulose paper using the transblot machine. Make sure to keep the orientation. | Transfer the gel to nitrocellulose paper using the transblot machine. Make sure to keep the orientation. |
Revision as of 09:36, 29 February 2016
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02/22/2016Ran an SDS protein gel with KAH following this protocol Gel lanes
Gel/ Buffer
Remove the gel from the box, rinse with DI water, scrape the exposed gel slit on the bottom to get all the gel from that spot. Rinse the gel gunk away. Crack the plastic apart but don't pull it apart until the plastic is unstuck. With the gel submerged in a tupperware filled with DI water, separate the plastic pieces. Pay attention to which plastic part the gel is sticking to. Pay attention to the direction of your gel. Get the gel floating in the DI water.
we had trouble getting it to start, had to move to B.
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