Haynes Lab:Notebook/CRISPR Editing/2016/02/26: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/02/26 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==02/26/2016== | ||
PCR of gB014 gblock with P169/216, gotaq mm, at=51degC, 30 cycles, two 100ul rxns, ran on gel, confirmed both rxns had bands at the correct length | |||
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Since the MV13-KAH60 digested and column purified backbone looked so badly on the nanodrop, I ran it on a gel. still looks like the majority of the DNA is at the correct size though I did see some bands at lower MW. | |||
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passaged Luc14s thawed yesterday, look good. | |||
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Revision as of 10:52, 27 February 2016
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02/26/2016PCR of gB014 gblock with P169/216, gotaq mm, at=51degC, 30 cycles, two 100ul rxns, ran on gel, confirmed both rxns had bands at the correct length
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