Haynes Lab:Notebook/CRISPR Editing/2016/02/27: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/02/27 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==02/27/2016== | ||
Finally found the FseI in the -80! Cut 1ug of pX330A_dCas9_1x4 vector from addgene and 80ng insert (gB009) with 1ul FseI for 1h at 37degC then deactivated at 65degC for 20min. Did three tubes of the backbone. | |||
:1ug bb | |||
:5ul cutsmart | |||
:1ul FseI | |||
:43ul H2O | |||
Revision as of 11:30, 27 February 2016
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02/27/2016Finally found the FseI in the -80! Cut 1ug of pX330A_dCas9_1x4 vector from addgene and 80ng insert (gB009) with 1ul FseI for 1h at 37degC then deactivated at 65degC for 20min. Did three tubes of the backbone.
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