Haynes Lab:Notebook/CRISPR Editing/2016/02/27: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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:5ul cutsmart | :5ul cutsmart | ||
:1ul FseI | :1ul FseI | ||
: | :40.41ul H2O | ||
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I didn't like that there were extra bands in the purified cut MV13-KAH60. Decide to redigest with less enzyme for shorter time. Cut 2ug of MV13-KAH60 vector with 0.3ul FseI for 18 min at 37degC then deactivated at 65degC for 20min. Did two tubes of the backbone. | |||
:2ug bb | |||
:5ul cutsmart | |||
:0.3ul FseI | |||
:38ul H2O | |||
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Revision as of 11:43, 27 February 2016
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02/27/2016Finally found the FseI in the -80! Cut 1ug of pX330A_dCas9_1x4 vector from addgene and 80ng insert (gB009) with 1ul FseI for 1h at 37degC then deactivated at 65degC for 20min. Did three tubes of the backbone.
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