Haynes Lab:Notebook/CRISPR Editing/2016/02/29: Difference between revisions
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
||
| Line 53: | Line 53: | ||
Gel/ Buffer | Gel/ Buffer | ||
:NuPAGE 4-12% BisTris | :NuPAGE 4-12% BisTris | ||
:NuPAGE® MOPS SDS Running Buffer<br> | :NuPAGE® MOPS SDS Running Buffer (20%MOPS diluted in DI water from sink)<br> | ||
remove the strip from the gel, put premade gel into box with the open part facing the outer chamber, fill inner chamber to cover the comb, outer chamber to cover the bottom of the gel.<br> | remove the strip from the gel, put premade gel into box with the open part facing the outer chamber, fill inner chamber to cover the comb, outer chamber to cover the bottom of the gel.<br> | ||
Add 500ul antioxidant to the inner chamber<br> | Add 500ul antioxidant to the inner chamber<br> | ||
| Line 61: | Line 61: | ||
load samples and ladder | load samples and ladder (5ul ladder)<br> | ||
:ladder in lanes 1 and 9 might have a bit less than 5ul<br> | |||
run gel for 1 hour at 120V (until lowest dye band ran just past the third ridge in gel box) | run gel for 1 hour at 120V (until lowest dye band ran just past the third ridge in gel box) | ||
Revision as of 08:38, 29 February 2016
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||
02/29/2015Running gel for western Gel lanes
Mastermix for sample buffer
heat at 100degC for 5 mins, cool in rack at RT
remove the strip from the gel, put premade gel into box with the open part facing the outer chamber, fill inner chamber to cover the comb, outer chamber to cover the bottom of the gel.
run gel for 1 hour at 120V (until lowest dye band ran just past the third ridge in gel box)
| |||||||||||||
