Haynes Lab:Notebook/CRISPR Editing/2016/02/29: Difference between revisions
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Should have no bands in the gel and bands on the nicrocellulose. Rinse the nitrocellulose in DI water. Move the nitrocellulose to a smaller dish. Pour Ponceau stain over to just cover it. Orbital shake gently for 5-10 minutes. Rinse the Ponceau in the sink in DI water until the background is white.<br> | Should have no bands in the gel and bands on the nicrocellulose. Rinse the nitrocellulose in DI water. Move the nitrocellulose to a smaller dish. Pour Ponceau stain over to just cover it. Orbital shake gently for 5-10 minutes. Rinse the Ponceau in the sink in DI water until the background is white.<br> |
Revision as of 10:24, 29 February 2016
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02/29/2015Plated Luc14s in 12-well plate, 0.1x10^6 cells/well, for transfections tomorrow Running gel for western Gel lanes
Mastermix for sample buffer
heat at 100degC for 5 mins, cool in rack at RT
remove the strip from the gel, put premade gel into box with the open part facing the outer chamber, fill inner chamber to cover the comb, outer chamber to cover the bottom of the gel.
run gel for 1 hour at 120V (until lowest dye band ran just past the third ridge in gel box)
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