Haynes Lab:Notebook/CRISPR Editing/2016/04/19: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/04/19 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==04/19/2016== | ||
Gal4-EED cells recently thawed and grown only in puro aren't growing well. I added new media with 20% FBS. | |||
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Neon transfection plate from yesterday was almost 100% confluent so i split into two 15cm plates. | |||
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expanded the gal4-eed+dox cells into four T175 flasks. Will be have enough to do all transfections in two days | |||
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ChIP with Josh! | |||
Maxiprep of g025, 31, 34 dCas9 plasmids. i froze bacterial pellets yesterday, thawed them and used the brafman lab macherey nagel maxi-prep kit | |||
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Revision as of 00:10, 20 April 2016
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04/19/2016Gal4-EED cells recently thawed and grown only in puro aren't growing well. I added new media with 20% FBS.
Maxiprep of g025, 31, 34 dCas9 plasmids. i froze bacterial pellets yesterday, thawed them and used the brafman lab macherey nagel maxi-prep kit | |
