05/30/2016
qPCR for ChIP!
Primer sets
| Primer set
|
Target
|
F primer
|
R primer
|
amplicon length
|
| 1 |
g025 |
|
|
|
| 2 |
g031, g034 |
P308 |
P160 |
202
|
| 3 |
GAPDH |
P203 |
P204 |
|
Plate 1
Primer master mix for primer sets 1 and 3
| '
|
1 wells
|
30
|
| 2x SYBR mix |
7.5 |
225
|
| 750nmol F/R primer mix |
3 |
90
|
| PCR water |
2.5 |
75
|
| total |
13 |
390
|
Primer master mix for primer set 2
| '
|
1 wells
|
22
|
| 2x SYBR mix |
7.5 |
165
|
| 750nmol F/R primer mix |
3 |
66
|
| PCR water |
2.5 |
55
|
| total |
13 |
286
|
Plate 2
Primer set 2
| '
|
1 wells
|
40
|
| 2x SYBR mix |
7.5 |
300
|
| 750nmol F/R primer mix |
3 |
120
|
| PCR water |
2.5 |
100
|
| total |
13 |
520
|
Plate 3
Primer set 3
| '
|
1 wells
|
40
|
| 2x SYBR mix |
7.5 |
300
|
| 750nmol F/R primer mix |
3 |
120
|
| PCR water |
2.5 |
100
|
| total |
13 |
520
|
Make the dilution curves for all three primer sets
Will use just Luc14 gDNA, maybe 10 dilution points in triplicate, will be 90
Redo nested PCR of the KAH228/g034 experiments
Did it with the wrong primers the first time, need to redo with P149/176.
Redo nested PCR of the Luc14 g034 samples
Did it with the wrong primers the first time, need to redo with P149/176. (need to double check with notebook to make sure I used 163/215).
| 
Project name
