Haynes Lab:Notebook/CRISPR Editing/2016/06/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2016/06/01 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==03//2015==
==06/01/2016==


'''Plate 5'''
<br>
L 25 F, I, set 3<br>
G 25 F pset 3<br>
Primer set 2 master mix<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''58'''
|-
| 2x SYBR mix||7.5||435
|-
| 750nmol F/R primer mix||3||174
|-
| PCR water||2.5||145
|-
| total||13||754
|}
<br>
Primer set 3 master mix<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''33'''
|-
| 2x SYBR mix||7.5||247.5
|-
| 750nmol F/R primer mix||3||99
|-
| PCR water||2.5||82.5
|-
| total||13||429
|}
<br>
[[Image:16.06.01 qPCR Plate 5 screen shot.png]]
<br>
<br>
'''Plate 6'''<br>
L 25 H pset 3<br>
L/G 31 F/I/input set 3
<br>
Primer set 3 master mix<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''69'''
|-
| 2x SYBR mix||7.5||517.5
|-
| 750nmol F/R primer mix||3||207
|-
| PCR water||2.5||172.5
|-
| total||13||897
|}
<br>
[[Image:16.06.01 qPCR Plate 6 screen shot.png]]




Revision as of 22:51, 31 May 2016

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

06/01/2016

Plate 5
L 25 F, I, set 3
G 25 F pset 3

Primer set 2 master mix

' 1 well 58
2x SYBR mix 7.5 435
750nmol F/R primer mix 3 174
PCR water 2.5 145
total 13 754


Primer set 3 master mix

' 1 well 33
2x SYBR mix 7.5 247.5
750nmol F/R primer mix 3 99
PCR water 2.5 82.5
total 13 429





Plate 6
L 25 H pset 3
L/G 31 F/I/input set 3


Primer set 3 master mix

' 1 well 69
2x SYBR mix 7.5 517.5
750nmol F/R primer mix 3 207
PCR water 2.5 172.5
total 13 897





Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2016/06/01&oldid=942168"