Haynes Lab:Notebook/CRISPR Editing/2016/06/16: Difference between revisions

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|-
|-
| total||13||754
| total||13||754
|}
<br><br>
'''Plate 11'''<br>
[[Image:16.06.16 qPCR plate 11 screen shot.png]]
<br>
P313/311 master mix<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''51'''
|-
| 2x SYBR mix||7.5||382.5
|-
| 750nmol F/R primer mix||3||153
|-
| PCR water||2.5||127.5
|-
| total||13||663
|}
Primer set 3 master mix <br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1 well'''
| align="center" style="background:#f0f0f0;"|'''27'''
|-
| 2x SYBR mix||7.5||202.5
|-
| 750nmol F/R primer mix||3||81
|-
| PCR water||2.5||67.5
|-
| total||13||351
|}
|}


Revision as of 14:57, 16 June 2016

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06/16/2016

Plate 10



P313/311 master mix

' 1 well 40
2x SYBR mix 7.5 300
750nmol F/R primer mix 3 120
PCR water 2.5 100
total 13 520


Pset 3 master mix

' 1 well 58
2x SYBR mix 7.5 435
750nmol F/R primer mix 3 174
PCR water 2.5 145
total 13 754



Plate 11


P313/311 master mix

' 1 well 51
2x SYBR mix 7.5 382.5
750nmol F/R primer mix 3 153
PCR water 2.5 127.5
total 13 663

Primer set 3 master mix

' 1 well 27
2x SYBR mix 7.5 202.5
750nmol F/R primer mix 3 81
PCR water 2.5 67.5
total 13 351


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