Haynes Lab:Notebook/CRISPR Editing/2016/08/29: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2016/08/29 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==08/29/2016== | ||
Sonicated Gal4+puro cells fixed from a T-175 flask, had about 100ul of cells, brought up to the higher volumes for cell lysis. Sonicated for 3 rounds of 5 cycles of 15s on, 45 off, adding ice between each round. Took 100ul to check efficiency, put at 65degC. Snap froze the rest and put at -80. | |||
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Checked concentration of sonicated chromatin, ~380ng/ul. Should have plenty to do the pulldowns in triplicate. | |||
Revision as of 15:13, 30 August 2016
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08/29/2016Sonicated Gal4+puro cells fixed from a T-175 flask, had about 100ul of cells, brought up to the higher volumes for cell lysis. Sonicated for 3 rounds of 5 cycles of 15s on, 45 off, adding ice between each round. Took 100ul to check efficiency, put at 65degC. Snap froze the rest and put at -80.
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