Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/01

From OpenWetWare

Jump to: navigation, search
(Entry title)
Current revision (19:15, 28 May 2013) (view source)
(Entry title)
 
Line 11: Line 11:
{| {{table}}
{| {{table}}
|-
|-
-
| Part number || Description || Part size (bp) || Backbone || Colonies
+
| Number||Part number || Description || Part size (bp) || Backbone || Colonies
|-  
|-  
| || || || || 1 || 2 || 3 || 4 || 5
| || || || || 1 || 2 || 3 || 4 || 5
|-
|-
-
| F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0
+
| 1||F2620 || p(tetR)-RBS-luxR-Stop-lux pR || 1061 || pSB1A2 || 0 || 0 || X || X || 0
|-
|-
-
| K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X  
+
| 2||K082035 || p(tetR)-RBS-rhlI-Stop-Stop || 884 || pSB1A2 || X || X  
|-  
|-  
-
| R0071 || RhlR-activated promoter || 53 || pSB1A2 || X ||   
+
| 3||R0071 || RhlR-activated promoter || 53 || pSB1A2 || X ||   
|-
|-
-
| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X  
+
|4|| K084007 || LacI-RBS-LasI || 835 || pSB1AK3 || X || X  
|-
|-
-
| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X  
+
|5|| K081016 || RBS-lasI-Stop || 735 || pSB1AK3 || 0 || X  
|-
|-
-
| K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X
+
| 6||K081023 || RBS-cl lam-RBS-lasR-Stop || 1653 || pSB1AK3 || X || 0 || X
|}
|}
Line 35: Line 35:
{| {{table}}
{| {{table}}
|-
|-
-
| Part number || Description || Part size (bp) || Backbone  
+
| Number ||Part number || Description || Part size (bp) || Backbone  
|-
|-
-
|K145254 ||Plac+RBS+LuxI+Stop+Stop|| 862 ||pSB1A2
+
|7||K145254 ||Plac+RBS+LuxI+Stop+Stop|| 862 ||pSB1A2
|-
|-
-
|K091134 || Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S ||2048 ||pSB1AK3
+
|8||K091134 || Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S ||2048 ||pSB1AK3
|-
|-
-
|K092100 || Pconst+RBS+RhlR+S+S|| 930 || pSB1A2
+
|9||K092100 || Pconst+RBS+RhlR+S+S|| 930 || pSB1A2
|-
|-
-
|K092900 || Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac ||2102 || pSB1A2
+
|10||K092900 || Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac ||2102 || pSB1A2
|-
|-
-
|E0240 || RBS+GFP+S+S ||876 || pSB1A2
+
|11||E0240 || RBS+GFP+S+S ||876 || pSB1A2
|}
|}

Current revision

Project name Main project page
Previous entry      Next entry

Entry title

Miniprepped the colonies grown up yesterday

NumberPart number Description Part size (bp) Backbone Colonies
1 2 3 4 5
1F2620 p(tetR)-RBS-luxR-Stop-lux pR 1061 pSB1A2 0 0 X X 0
2K082035 p(tetR)-RBS-rhlI-Stop-Stop 884 pSB1A2 X X
3R0071 RhlR-activated promoter 53 pSB1A2 X
4 K084007 LacI-RBS-LasI 835 pSB1AK3 X X
5 K081016 RBS-lasI-Stop 735 pSB1AK3 0 X
6K081023 RBS-cl lam-RBS-lasR-Stop 1653 pSB1AK3 X 0 X

Also tried a second elution on the miniprep columns using 50ul water. Got pretty decent yields from that but need to confirm on a gel that it's a good idea.


Pulled more parts out of the registry

Number Part number Description Part size (bp) Backbone
7K145254 Plac+RBS+LuxI+Stop+Stop 862 pSB1A2
8K091134 Plac+RBS+LasR+S+S+PlasR+RBS+GFP+S+S 2048 pSB1AK3
9K092100 Pconst+RBS+RhlR+S+S 930 pSB1A2
10K092900 Pconst+RBS+RhlR+S+S+PrhlR+RBS+lac 2102 pSB1A2
11E0240 RBS+GFP+S+S 876 pSB1A2



Personal tools