Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Entry title== | ==Entry title== | ||
Did tons of minipreps, many of them did not work well. | |||
Probably picked colonies from the new plates | |||
Cut some parts and ran a gel of cut plasmids: | |||
[[image:13.03.02_edit.jpg |400px|digests]] | |||
{| {{table}} | |||
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|Lane label || Part number || Restriction enzymes||expected length | |||
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|1||F2620|| X & P|| 1061 & 2079 | |||
|- | |||
|2||constitutive promoter||S & P ||2000ish | |||
|- | |||
|3||constitutive promoter w RFP||S&P||2000ish | |||
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|4|| R0071 (probably) || S & P || 3029 | |||
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|5||K081016|| X & P || 735 & 2079 | |||
|- | |||
||6|| K081023|| X & P || 1653 & 2079 | |||
|} | |||
Latest revision as of 22:32, 26 September 2017
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Entry titleDid tons of minipreps, many of them did not work well. Probably picked colonies from the new plates Cut some parts and ran a gel of cut plasmids:
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