Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/02

Current revision

Project name

Probably picked colonies from the new plates

Cut some parts and ran a gel of cut plasmids:

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+Probably picked colonies from the new plates
+Cut some parts and ran a gel of cut plasmids:
+[[image:13.03.02_edit.jpg‎ |400px|digests]]
+{| {{table}}
+|-
+|Lane label || Part number || Restriction enzymes||expected length
+|-
+|1||F2620|| X & P|| 1061 & 2079
+|-
+|2||constitutive promoter||S & P ||2000ish
+|-
+|3||constitutive promoter w RFP||S&P||2000ish
+|-
+|4|| R0071 (probably) || S & P || 3029
+|-
+|5||K081016|| X & P || 735 & 2079
+|-
+||6|| K081023|| X & P || 1653 & 2079
+|}