Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/02: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/02 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
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==Entry title== | ==Entry title== | ||
Did tons of minipreps, many of them did not work well. | |||
Probably picked colonies from the new plates | |||
Cut some parts and ran a gel of cut plasmids: | |||
[[image:13.03.02_edit.jpg |400px|digests]] | |||
{| {{table}} | |||
|- | |||
|Lane label || Part number || Restriction enzymes||expected length | |||
|- | |||
|1||F2620|| X & P|| 1061 & 2079 | |||
|- | |||
|2||constitutive promoter||S & P ||2000ish | |||
|- | |||
|3||constitutive promoter w RFP||S&P||2000ish | |||
|- | |||
|4|| R0071 (probably) || S & P || 3029 | |||
|- | |||
|5||K081016|| X & P || 735 & 2079 | |||
|- | |||
||6|| K081023|| X & P || 1653 & 2079 | |||
|} | |||
Revision as of 16:15, 28 May 2013
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Entry titleDid tons of minipreps, many of them did not work well. Probably picked colonies from the new plates Cut some parts and ran a gel of cut plasmids:
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