Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/07: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2013/03/07 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs) |
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==Entry title== | ==Entry title== | ||
minipreps | |||
streak | |||
cut and extract and clone | |||
pull out promoters + RBS from registry | |||
gel extract and ligate and transform part 6 | |||
miniprep | |||
C0061 1 and 2 | |||
C0062 | |||
K081016 that haynes grew up, streaked on plate (she found by PCR) | |||
R0071 that haynes grew up, streaking on plate " " | |||
cut K081016 and R0071 from above, ran on a gel, didn't work | |||
Ernesto ran colony PCR on a bunch of mini preps and colonies | |||
transformed RBS+LuxI and RBS+LasI | |||
Revision as of 13:29, 28 May 2013
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Entry titleminipreps streak cut and extract and clone pull out promoters + RBS from registry gel extract and ligate and transform part 6
cut K081016 and R0071 from above, ran on a gel, didn't work Ernesto ran colony PCR on a bunch of mini preps and colonies transformed RBS+LuxI and RBS+LasI
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