Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/08

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(Autocreate 2013/03/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (16:40, 28 May 2013) (view source)
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==Entry title==
==Entry title==
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* Insert content here...
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Ran the colony PCRs Ernesto ran yesterday on a gel:
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[[image:13.03.08_colony_PCR_(Ernesto)_edit.jpg‎|400px|colony PCR]]
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{| {{table}}
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|-
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|Label||Part #|| Part length|| Expected band length
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|-
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|plate 2|| K082035|| 884bp||1134bp
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|-
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|plate 4||K084007 ||835bp||1085bp
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|-
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|plate 5|| K081016|| 735bp||985bp
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|-
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|plate 7|| K145254|| 862bp||1112bp
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|}
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minipreps:
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{| {{table}}
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|-
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|Label||Part #|| colony|| part length || expected band length
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|-
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|3-k||R0071 ||picked by KAH ||53bp || 300bp
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|-
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|5-k||K081016|| picked by KAH|| 735bp||1035bp
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|-
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|2-2|| K082035 ||colony 2 ||884bp||1134bp
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|-
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|4-2|| K084007 ||colony 2 ||835bp||1085bp
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|-
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|7-1|| K145254 ||colony 1 ||862bp||1112bp
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|-
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|7-3|| K145254|| colony 3|| 862bp||1112bp
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|}
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<br>
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Minipreps:
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{| {{table}}
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|-
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|Part||260||280||260/280|| ng/ul
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|-
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|B0034+C0061||0.042||0.022||1.873||41.772
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|-
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|B0034+C0061||0.043||0.023||1.866||43.094
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|-
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|B0034+C0078||0.061||0.031||1.93||60.539
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|-
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|B0034+C0078||0.031||0.017||1.832||31.39
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|-
|}
|}
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<br>
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Gel extractions:
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{| {{table}}
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|-
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|Part||260||280||260/280|| ng/ul
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|-
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|RBS+LuxI X/P||0.004||0.001||3.545||4.104
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|-
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|RBS+LasI X/P||0.002||0.001||2.125||1.809
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|-
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|R0071 S/P||0.006||0.003||2.069||6.317
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|-
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|K081023 X/P||0.025||0.014||1.859||25.325
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|-
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|}
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__NOTOC__
__NOTOC__

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Entry title

Ran the colony PCRs Ernesto ran yesterday on a gel:

colony PCR

LabelPart # Part length Expected band length
plate 2 K082035 884bp1134bp
plate 4K084007 835bp1085bp
plate 5 K081016 735bp985bp
plate 7 K145254 862bp1112bp

minipreps:

LabelPart # colony part length expected band length
3-kR0071 picked by KAH 53bp 300bp
5-kK081016 picked by KAH 735bp1035bp
2-2 K082035 colony 2 884bp1134bp
4-2 K084007 colony 2 835bp1085bp
7-1 K145254 colony 1 862bp1112bp
7-3 K145254 colony 3 862bp1112bp


Minipreps:

Part260280260/280 ng/ul
B0034+C00610.0420.0221.87341.772
B0034+C00610.0430.0231.86643.094
B0034+C00780.0610.0311.9360.539
B0034+C00780.0310.0171.83231.39


Gel extractions:

Part260280260/280 ng/ul
RBS+LuxI X/P0.0040.0013.5454.104
RBS+LasI X/P0.0020.0012.1251.809
R0071 S/P0.0060.0032.0696.317
K081023 X/P0.0250.0141.85925.325



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