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Entry title
Ran the colony PCRs Ernesto ran yesterday on a gel:
Label |
Part # |
Part length |
Expected band length
|
plate 2 |
K082035 |
884bp |
1134bp
|
plate 4 |
K084007 |
835bp |
1085bp
|
plate 5 |
K081016 |
735bp |
985bp
|
plate 7 |
K145254 |
862bp |
1112bp
|
minipreps:
Label |
Part # |
colony |
part length |
expected band length
|
3-k |
R0071 |
picked by KAH |
53bp |
300bp
|
5-k |
K081016 |
picked by KAH |
735bp |
1035bp
|
2-2 |
K082035 |
colony 2 |
884bp |
1134bp
|
4-2 |
K084007 |
colony 2 |
835bp |
1085bp
|
7-1 |
K145254 |
colony 1 |
862bp |
1112bp
|
7-3 |
K145254 |
colony 3 |
862bp |
1112bp
|
Minipreps:
Part |
260 |
280 |
260/280 |
ng/ul
|
B0034+C0061 |
0.042 |
0.022 |
1.873 |
41.772
|
B0034+C0061 |
0.043 |
0.023 |
1.866 |
43.094
|
B0034+C0078 |
0.061 |
0.031 |
1.93 |
60.539
|
B0034+C0078 |
0.031 |
0.017 |
1.832 |
31.39
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Gel extractions:
Minipreps:
Part |
260 |
280 |
260/280 |
ng/ul
|
RBS+LuxI X/P |
0.004 |
0.001 |
3.545 |
4.104
|
RBS+LasI X/P |
0.002 |
0.001 |
2.125 |
1.809
|
R0071 S/P |
0.006 |
0.003 |
2.069 |
6.317
|
K081023 X/P |
0.025 |
0.014 |
1.859 |
25.325
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