Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/08

From OpenWetWare
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

Ran the colony PCRs Ernesto ran yesterday on a gel:

colony PCR

Label Part # Part length Expected band length
plate 2 K082035 884bp 1134bp
plate 4 K084007 835bp 1085bp
plate 5 K081016 735bp 985bp
plate 7 K145254 862bp 1112bp

minipreps:

Label Part # colony part length expected band length
3-k R0071 picked by KAH 53bp 300bp
5-k K081016 picked by KAH 735bp 1035bp
2-2 K082035 colony 2 884bp 1134bp
4-2 K084007 colony 2 835bp 1085bp
7-1 K145254 colony 1 862bp 1112bp
7-3 K145254 colony 3 862bp 1112bp


Minipreps:

Part 260 280 260/280 ng/ul
B0034+C0061 0.042 0.022 1.873 41.772
B0034+C0061 0.043 0.023 1.866 43.094
B0034+C0078 0.061 0.031 1.93 60.539
B0034+C0078 0.031 0.017 1.832 31.39


Gel extractions:

Part 260 280 260/280 ng/ul
RBS+LuxI X/P 0.004 0.001 3.545 4.104
RBS+LasI X/P 0.002 0.001 2.125 1.809
R0071 S/P 0.006 0.003 2.069 6.317
K081023 X/P 0.025 0.014 1.859 25.325



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2013/03/08&oldid=700114"