Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/09

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(Autocreate 2013/03/09 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (22:29, 9 March 2013) (view source)
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==Entry title==
==Entry title==
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* Insert content here...
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miniprepped RBS+LuxI and RBS+LasI<br>
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cut (all minipreps, some from before, colony numbers on gel) for 15 minutes at 37deg and ran a gel right away:
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[[image: ‎ |400px|digests]]
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{| {{table}}
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|-
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|Lane label || Part number || Restriction enzymes||expected length
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|-
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|1||B0034+C0061|| X & P|| 661 & 2000ish
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|-
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|2||cB0034+C0078|| X & P ||685 & 2000ish
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|-
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|3||K082035 ||X&P||884 & 2000ish
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|-
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|4|| K084007 || X & P || 835 & 2000ish
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|-
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|5||K145254|| X & P || 862 & 2079
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|-
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|6|| K092100|| S & P || 2909ish
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|-
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|7||K091134|| X & P || 2048 & 2000ish
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|}
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Entry title

miniprepped RBS+LuxI and RBS+LasI
cut (all minipreps, some from before, colony numbers on gel) for 15 minutes at 37deg and ran a gel right away:

[[image: ‎ |400px|digests]]

Lane label Part number Restriction enzymesexpected length
1B0034+C0061 X & P 661 & 2000ish
2cB0034+C0078 X & P 685 & 2000ish
3K082035 X&P884 & 2000ish
4 K084007 X & P 835 & 2000ish
5K145254 X & P 862 & 2079
6 K092100 S & P 2909ish
7K091134 X & P 2048 & 2000ish


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