Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/03/09

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Current revision (23:29, 9 March 2013) (view source)
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==Entry title==
==Entry title==
miniprepped RBS+LuxI and RBS+LasI<br>
miniprepped RBS+LuxI and RBS+LasI<br>
-
cut and ran a gel:
+
cut (all minipreps, some from before, colony numbers on gel) for 15 minutes at 37deg and ran a gel right away:
[[image: ‎ |400px|digests]]
[[image: ‎ |400px|digests]]

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miniprepped RBS+LuxI and RBS+LasI
cut (all minipreps, some from before, colony numbers on gel) for 15 minutes at 37deg and ran a gel right away:

[[image: ‎ |400px|digests]]

Lane label Part number Restriction enzymesexpected length
1B0034+C0061 X & P 661 & 2000ish
2cB0034+C0078 X & P 685 & 2000ish
3K082035 X&P884 & 2000ish
4 K084007 X & P 835 & 2000ish
5K145254 X & P 862 & 2079
6 K092100 S & P 2909ish
7K091134 X & P 2048 & 2000ish


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