Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/23: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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|2|| F2620-J06702 || Amp|| DH5aT||50ul||Fast | |2|| F2620-J06702 || Amp|| DH5aT||50ul||Fast | ||
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|3||TraI (from stef)||Kan||DH5aT||Long | |3||TraI (from stef)||Kan||DH5aT||30||Long | ||
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|4||E0240 m.p 3/3/13 111ng/ul ||Amp||DH5aT||30ul||Fast | |4||E0240 m.p 3/3/13 111ng/ul ||Amp||DH5aT||30ul||Fast |
Revision as of 12:48, 28 May 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Entry titleMiniprepped colonies picked yesterday
Digests:
'*Not totally sure what the backbone length is. Because of the low mini prep yields for 1-4, I used more DNA for digests 1-4:
For digest 5, I made a lower reaction volume because it's only to confirm:
Digests ran for 20 min at 37deg
Ligation reactions ran for 15min at room temp. Agitated occasionally.
For 3 and 5, iced for 5min, heat shock for 45s at 42deg, iced for 2 min. Plated 5, shook 3 with 800ul SOC for 30min then plated. |