Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/23: Difference between revisions
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|8||K092900 mp 4/1/13 12ng/ul (EL)||Amp||DH5aT||30ul||Fast | |8||K092900 mp 4/1/13 12ng/ul (EL)||Amp||DH5aT||30ul||Fast | ||
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For 3 and 5, iced for 5min, heat shock for 45s at 42deg, iced for 2 min. Plated 5, shook 3 with 800ul SOC for 30min then plated. | For 3 and 5, iced for 5min, heat shock for 45s at 42deg, iced for 2 min. Plated 5, shook 3 with 800ul SOC for 30min then plated. |
Revision as of 12:49, 28 May 2013
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Entry titleMiniprepped colonies picked yesterday
Digests:
'*Not totally sure what the backbone length is. Because of the low mini prep yields for 1-4, I used more DNA for digests 1-4:
For digest 5, I made a lower reaction volume because it's only to confirm:
Digests ran for 20 min at 37deg
Ligation reactions ran for 15min at room temp. Agitated occasionally.
For 3 and 5, iced for 5min, heat shock for 45s at 42deg, iced for 2 min. Plated 5, shook 3 with 800ul SOC for 30min then plated. |