Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/30

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(Autocreate 2013/05/30 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
(Entry title)
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==Entry title==
==Entry title==
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* Insert content here...
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9AM
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BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.  
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<br>
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Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.  
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<br>
 +
<br>
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Co-cultures:
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Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.
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{| {{table}}
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|-
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|Sender||Receiver
 +
|-
 +
|LuxI||LuxR
 +
|-
 +
|LasI||LuxR
 +
|-
 +
|RhlI||LuxR
 +
|-
 +
|TraI||LuxR
 +
|-
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|TraR ctrl||LuxR
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|-
 +
}}
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<br><br>
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Supernatant transfer:
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Determine pH using test strips:
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{| {{table}}
 +
|-
 +
|Sender||pH
 +
|-
 +
|LuxI||
 +
|-
 +
|LasI||
 +
|-
 +
|RhlI||
 +
|-
 +
|TraI||
 +
|-
 +
|TraR ctrl||
 +
|-
 +
|}
 +
 
 +
 

Revision as of 11:53, 30 May 2013

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Entry title

9AM

BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.
Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.

Co-cultures: Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.

}}

Supernatant transfer: Determine pH using test strips:
SenderReceiver
LuxILuxR
LasILuxR
RhlILuxR
TraILuxR
TraR ctrlLuxR
SenderpH
LuxI
LasI
RhlI
TraI
TraR ctrl




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