Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/30

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Revision as of 12:54, 30 May 2013

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9AM

BL21 sender cultures grew in 6mls and 2mls. RhlR-GFP still didn't grow.
Shook LuxR, TraI, and TraR that had been in the fridge for a few minutes.

Co-cultures: Add 100ul of Sender, 100ul of Receiver to 3ml LB+Amp (except for TraI culture, which will not have antibiotic). Started shaking at 10AM.

SenderReceiver
LuxILuxR
LasILuxR
RhlILuxR
TraILuxR
TraR ctrlLuxR



Supernatant transfer: Determine pH using test strips:

SenderpH
LuxI
LasI
RhlI
TraI
TraR ctrl




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