Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/05/31

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Current revision (14:57, 31 May 2013) (view source)
(05/31/2013)
 
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==05/31/2013==
==05/31/2013==
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9AM
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9AM<br>
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Spun 2ml of supernatant transfer experiments and 1ml co-culture experiments in 2ml tubes at full speed for 3 minutes. Poured off supernatant, resuspended cells in 100ul water. Could see that some of the cell pellets were green while controls were not. Moved resuspended cells to plate for fluorescence reading. Added 100ul of each supernatant culture to plate as well for cell density readings.
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<br><br>
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9:30AM <br>
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Put the sup experiments that were left at 4deg back into round bottom tubes and started shaking again.

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05/31/2013

9AM
Spun 2ml of supernatant transfer experiments and 1ml co-culture experiments in 2ml tubes at full speed for 3 minutes. Poured off supernatant, resuspended cells in 100ul water. Could see that some of the cell pellets were green while controls were not. Moved resuspended cells to plate for fluorescence reading. Added 100ul of each supernatant culture to plate as well for cell density readings.

9:30AM
Put the sup experiments that were left at 4deg back into round bottom tubes and started shaking again.



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