Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/19: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br> | Started the oligo annealing over again. I shouldn't have cut or dephosphorylated the oligos. That was dumb. <br> | ||
Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields. | Miniprepped the colonies I picked last night. Tried splitting the 3ml cultures into 2-1.5ml cultures to mini prep. Still got low yields. | ||
{|table | {| {{table}} | ||
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|Part||Size||Miniprep yield 1||Miniprep yield 2 | |Part||Size||Miniprep yield 1||Miniprep yield 2 |
Revision as of 17:07, 19 July 2013
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07/19/2013Ligation/transformation plates have colonies but they're all in the middle. Same with the backbone control. Picked colonies just in case.
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