Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/07/20

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(07/20/2013)
Current revision (23:52, 21 July 2013) (view source)
(07/20/2013)
 
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==07/20/2013==
==07/20/2013==
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All the  
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All the plasmid transformations looked good except for K091134, which was expected. Picked colonies from all of them. <br>
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{| {{table}}
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Oligo ligation-transformations didn't look great but I picked a couple colonies.<br><br<
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Came back in the evening, ligation colonies, K091134, and K092900+GFP didn't grow. Left the plates on the bench to see if they would grow larger colonies.<br>
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|No. || Name||Length||Gel check
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spun down the other liquid cultures and put at -20 because we're low on mini prep columns.
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|1||LuxR-GFP Receiver|| 1900|| +
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|2||K092900 + E0240||2200||-
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|3 ||C0061 - 1||643||couldn't tell
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|4||C0061 - 2||643||couldn't tell
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|5||C0062||781||couldn't tell
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|6||F2620 (81ng/ul)||1061||+
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|7||F2620 (52ng/ul)||1061||+
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|8||K091134||2048||-
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|9||K092900||2102||_
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|10||K145254||862||+
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|11||K082035||884||+
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|12||I0466||942||+
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|13||K084007||835||+
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|14||RBS-LasI||600||+
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|15||messed up||?||-
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|16||K092900-cyan||2900||-
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|17||F2620-GFP||2000||-
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|18||K084007||835||+
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|19||RBS-LuxI||798||not sure
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|20||J06702 56ng/ul||869||+
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07/20/2013

All the plasmid transformations looked good except for K091134, which was expected. Picked colonies from all of them.
Oligo ligation-transformations didn't look great but I picked a couple colonies.
<br< Came back in the evening, ligation colonies, K091134, and K092900+GFP didn't grow. Left the plates on the bench to see if they would grow larger colonies.
spun down the other liquid cultures and put at -20 because we're low on mini prep columns.



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