Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/09/28: Difference between revisions

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(Autocreate 2013/09/28 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
'''8am'''
 
{| {{table}}
|-
|Sender/Receiver||OD650||GFP||RFP
|-
|EsaI||0.679||1500||
|-
| || 0.671||1545||
|-
| || 0.634||1402||
|-
|RhlI||0.755||13497||
|-
| || 0.792||14323||
|-
| || 0.763||13992||
|-
|Ctrl||0.736||6645||
| ||0.712||6485||
|-
| ||0.740||6598||
|-
|LuxR-GFP||0.548||1619||
|-
|||0.563||1858||
|-
|||0.576||1957||
|-
|LuxR-RFP||0.576|| || 768
|-
| ||0.564 || ||682
|-
| ||0.562|| ||694
|-
|}
<br>
Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
<br>
spun receiver cells in 2mL tubes at 12k g for 5min


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Revision as of 16:16, 14 October 2013

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mm/dd/yyyy

8am

Sender/Receiver OD650 GFP RFP
EsaI 0.679 1500
0.671 1545
0.634 1402
RhlI 0.755 13497
0.792 14323
0.763 13992
Ctrl 0.736 6645 0.712 6485
0.740 6598
LuxR-GFP 0.548 1619
0.563 1858
0.576 1957
LuxR-RFP 0.576 768
0.564 682
0.562 694


Combined 3-5mL cultures of each sender into 15mL conicals. Spun in Wang lab large centrifuge
spun receiver cells in 2mL tubes at 12k g for 5min