Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/08: Difference between revisions

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(Autocreate 2013/10/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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PCR of RhlI with new sender/pLux primers<br>
50ul reactions
:pLux vector F/R
:Sender GG 2 F/R
 
Used RhlI in I13522 from 8/31/13 as template <br>
'''Thought maybe this would help because this would space out where the two primers should bind. Realize that it won't work for primers adding an RBS because the F primer won't bind'''





Revision as of 15:43, 15 October 2013

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mm/dd/yyyy

PCR of RhlI with new sender/pLux primers
50ul reactions

pLux vector F/R
Sender GG 2 F/R

Used RhlI in I13522 from 8/31/13 as template
Thought maybe this would help because this would space out where the two primers should bind. Realize that it won't work for primers adding an RBS because the F primer won't bind