Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/08

From OpenWetWare

Jump to: navigation, search
(Autocreate 2013/10/08 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (18:43, 15 October 2013) (view source)
(mm/dd/yyyy)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==mm/dd/yyyy==
==mm/dd/yyyy==
-
* Insert content here...
+
PCR of RhlI with new sender/pLux primers<br>
 +
50ul reactions
 +
:pLux vector F/R
 +
:Sender GG 2 F/R
 +
 
 +
Used RhlI in I13522 from 8/31/13 as template <br>
 +
'''Thought maybe this would help because this would space out where the two primers should bind. Realize that it won't work for primers adding an RBS because the F primer won't bind'''

Current revision

Project name Main project page
Previous entry      Next entry

mm/dd/yyyy

PCR of RhlI with new sender/pLux primers
50ul reactions

pLux vector F/R
Sender GG 2 F/R

Used RhlI in I13522 from 8/31/13 as template
Thought maybe this would help because this would space out where the two primers should bind. Realize that it won't work for primers adding an RBS because the F primer won't bind



Personal tools