Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/09: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
||
Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==mm/dd/yyyy== | ==mm/dd/yyyy== | ||
'''Cloning''' | '''Cloning'''<br> | ||
Cut 10ul J06702 (131) with E/X in 20ul reaction<br> | Cut 10ul J06702 (131) with E/X in 20ul reaction<br> | ||
Cut Gblock pLac-RBS with E/S<br> | Cut Gblock pLac-RBS with E/S<br> | ||
Line 44: | Line 44: | ||
heat shock at 42°C for 30s<br> | heat shock at 42°C for 30s<br> | ||
ice 2min<br><br> | ice 2min<br><br> | ||
'''GG PCR''' | '''GG PCR'''<br> | ||
Ran PCR from 10/8 on gel<br> | Ran PCR from 10/8 on gel<br> | ||
Reporter (pLux) primers worked, no background<br> | Reporter (pLux) primers worked, no background<br> |
Revision as of 15:57, 15 October 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||
mm/dd/yyyyCloning
Insert:backbone ratios 1:1, 2:1, 3:1 }} Incubate at RT for 35minAdd 40ul DH5αT cells to entire rxn Incubate on ice for 30 mins heat shock at 42°C for 30s ice 2min GG PCR Ran PCR from 10/8 on gel Reporter (pLux) primers worked, no background Sender did not work. Retried with I13522-2
|