Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/10

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(Autocreate 2013/10/10 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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Spun all cells in 15mL conicals at 2000RCF<br>
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Combined triplicates<br>
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{| {{table}}
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|-
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| ||OD650||GFP
 +
|-
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|LuxR 1||0.773||0.7963
 +
|-
 +
|LuxR 2||0.768||8088
 +
|-
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|RhlI||0.706||1579
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|-
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|EsaI||0.792||2597
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|-
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|}
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<br>
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Resuspended 30mL culuter into 3mL total
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:1.5mLResidual sup+pellet
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:1.5mL=new LB+amp
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::Measured by pipetting into a new tube
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<br>
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1mL=100uL, add 300ul into each tube
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<br>
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'''2:30PM'''<br>
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Started shaking
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<br><br>
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Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
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<br>
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'''7PM'''
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<br><br>
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'''8PM'''
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<br><br>
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'''9PM'''
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<br><br>
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'''10PM'''
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<br><br>
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'''11:30PM'''
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<br><br>
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<br><br>
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'''another one tomorrow at 10AM'''
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Ligation pLac-RBS-into J06702
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:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?
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Revision as of 19:59, 21 October 2013

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mm/dd/yyyy

Spun all cells in 15mL conicals at 2000RCF
Combined triplicates

OD650GFP
LuxR 10.7730.7963
LuxR 20.7688088
RhlI0.7061579
EsaI0.7922597


Resuspended 30mL culuter into 3mL total

1.5mLResidual sup+pellet
1.5mL=new LB+amp
Measured by pipetting into a new tube


1mL=100uL, add 300ul into each tube
2:30PM
Started shaking

Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM

8PM

9PM

10PM

11:30PM



another one tomorrow at 10AM Ligation pLac-RBS-into J06702

tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?


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