Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/10: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/10/10 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
Spun all cells in 15mL conicals at 2000RCF<br>
 
Combined triplicates<br>
 
{| {{table}}
|-
| ||OD650||GFP
|-
|LuxR 1||0.773||0.7963
|-
|LuxR 2||0.768||8088
|-
|RhlI||0.706||1579
|-
|EsaI||0.792||2597
|-
|}
<br>
Resuspended 30mL culuter into 3mL total
:1.5mLResidual sup+pellet
:1.5mL=new LB+amp
::Measured by pipetting into a new tube
<br>
1mL=100uL, add 300ul into each tube
<br>
'''2:30PM'''<br>
Started shaking
<br><br>
Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
<br>
'''7PM'''
<br><br>
'''8PM'''
<br><br>
'''9PM'''
<br><br>
'''10PM'''
<br><br>
'''11:30PM'''
<br><br>
<br><br>
'''another one tomorrow at 10AM'''
Ligation pLac-RBS-into J06702
:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 17:59, 21 October 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

mm/dd/yyyy

Spun all cells in 15mL conicals at 2000RCF
Combined triplicates

OD650 GFP
LuxR 1 0.773 0.7963
LuxR 2 0.768 8088
RhlI 0.706 1579
EsaI 0.792 2597


Resuspended 30mL culuter into 3mL total

1.5mLResidual sup+pellet
1.5mL=new LB+amp
Measured by pipetting into a new tube


1mL=100uL, add 300ul into each tube
2:30PM
Started shaking

Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM

8PM

9PM

10PM

11:30PM



another one tomorrow at 10AM Ligation pLac-RBS-into J06702

tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs/2013/10/10&oldid=741565"