Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/10

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Current revision (20:00, 21 October 2013) (view source)
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'''another one tomorrow at 10AM'''
'''another one tomorrow at 10AM'''
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Ligation pLac-RBS-into J06702
Ligation pLac-RBS-into J06702
:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?  
:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?  

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Spun all cells in 15mL conicals at 2000RCF
Combined triplicates

OD650GFP
LuxR 10.7730.7963
LuxR 20.7688088
RhlI0.7061579
EsaI0.7922597


Resuspended 30mL culuter into 3mL total

1.5mLResidual sup+pellet
1.5mL=new LB+amp
Measured by pipetting into a new tube


1mL=100uL, add 300ul into each tube
2:30PM
Started shaking

Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM

8PM

9PM

10PM

11:30PM



another one tomorrow at 10AM

Ligation pLac-RBS-into J06702

tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?


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