Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/10: Difference between revisions

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'''another one tomorrow at 10AM'''
'''another one tomorrow at 10AM'''
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Ligation pLac-RBS-into J06702
Ligation pLac-RBS-into J06702
:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?  
:tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?  

Revision as of 18:00, 21 October 2013

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mm/dd/yyyy

Spun all cells in 15mL conicals at 2000RCF
Combined triplicates

OD650 GFP
LuxR 1 0.773 0.7963
LuxR 2 0.768 8088
RhlI 0.706 1579
EsaI 0.792 2597


Resuspended 30mL culuter into 3mL total

1.5mLResidual sup+pellet
1.5mL=new LB+amp
Measured by pipetting into a new tube


1mL=100uL, add 300ul into each tube
2:30PM
Started shaking

Took measurements every hour-ish by pulling 100ul from each tube and measuring GFP and OD650
7PM

8PM

9PM

10PM

11:30PM



another one tomorrow at 10AM

Ligation pLac-RBS-into J06702

tons of colonies on all plates, including backbone. Maybe because I didn't gel purify the vector, I could have single cut? Or uncut?


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