Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/15

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(Autocreate 2013/10/15 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
Current revision (20:05, 21 October 2013) (view source)
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Recut more J06702 for 55 minutes.  
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:Colonies on background plate likely from uncut vector. <br>
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Gel purified.<br>
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Also ran PCR products from yesterday<br>
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Gel results
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{| {{table}}
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|-
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|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5
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|-
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|Ladder|| LuxI|| LasI|| LasR|| pLas|| J06702
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|-
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| ||No band||1 correct band||1 correct band||no band||1 correct band
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|-
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|}
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<br>
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Extracted J06702 from gel. 44ng/μl.
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mm/dd/yyyy

Recut more J06702 for 55 minutes.

Colonies on background plate likely from uncut vector.

Gel purified.
Also ran PCR products from yesterday
Gel results

Lane 1Lane 2Lane 3Lane 4Lane 5
Ladder LuxI LasI LasR pLas J06702
No band1 correct band1 correct bandno band1 correct band


Extracted J06702 from gel. 44ng/μl.


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