Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/15: Difference between revisions

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(Autocreate 2013/10/15 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
Recut more J06702 for 55 minutes.  
 
:Colonies on background plate likely from uncut vector. <br>
Gel purified.<br>
Also ran PCR products from yesterday<br>
Gel results
{| {{table}}
|-
|Lane 1||Lane 2||Lane 3||Lane 4||Lane 5
|-
|Ladder|| LuxI|| LasI|| LasR|| pLas|| J06702
|-
| ||No band||1 correct band||1 correct band||no band||1 correct band
|-
|}
<br>
Extracted J06702 from gel. 44ng/μl.


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Revision as of 18:05, 21 October 2013

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mm/dd/yyyy

Recut more J06702 for 55 minutes.

Colonies on background plate likely from uncut vector.

Gel purified.
Also ran PCR products from yesterday
Gel results

Lane 1 Lane 2 Lane 3 Lane 4 Lane 5
Ladder LuxI LasI LasR pLas J06702
No band 1 correct band 1 correct band no band 1 correct band


Extracted J06702 from gel. 44ng/μl.


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