Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/18

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(Autocreate 2013/10/18 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
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==mm/dd/yyyy==
==mm/dd/yyyy==
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* Insert content here...
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Retry ligation with gel extracted J06702
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<br>
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Backbone: J06702 E/X: 44 ng/ul<br>
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:Cut, gel purified
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Insert: Gblck pLac-RBS E/S: 2.6ng/ul
 +
:Cut, column purified<br>
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"did a bunch of calculations, only actually tried 3:1 and bb ctrl''<br>
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{| {{table}}
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|-
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| ||1:1||2:1||3:1||Bb ctrl
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|-
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|insert||0.5||1.0||1.5||0
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|-
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|vector||1.13||1.13||1.13||1.13
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|-
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|2x Roche ligation buffer||5||5||5||5
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|-
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|T4 ligase||1||1||1||1
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|-
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|H20||2.37||1.87||1.37||2.87
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|-
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|}
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<br>
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ligations ran for 25min at RT
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<br>
 +
Incubate with 40ul DH5αT for 15 min on ice

Revision as of 20:10, 21 October 2013

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mm/dd/yyyy

Retry ligation with gel extracted J06702
Backbone: J06702 E/X: 44 ng/ul

Cut, gel purified

Insert: Gblck pLac-RBS E/S: 2.6ng/ul

Cut, column purified

"did a bunch of calculations, only actually tried 3:1 and bb ctrl

1:12:13:1Bb ctrl
insert0.51.01.50
vector1.131.131.131.13
2x Roche ligation buffer5555
T4 ligase1111
H202.371.871.372.87


ligations ran for 25min at RT
Incubate with 40ul DH5αT for 15 min on ice



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