Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/23: Difference between revisions

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(Autocreate 2013/10/23 Entry for Haynes_Lab:Notebook/Characterizing_AHL_quorum_sensing_homologs)
 
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==mm/dd/yyyy==
==mm/dd/yyyy==
* Insert content here...
miniprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.<br>
{| {{table}}
|-
|plasmid||260||280||260/280||concentration
|-
|J06702||0.247|| 0.128 ||1.93 ||247.2
|-
|ligation||0.071|| 0.035 ||2.006||70.9
|-
|}
<br>
 
Digested J06702 with
:E only for 10 minutes
:X only for 10 minutes
:E and X for 10 minutes
:E only for 5, then add X for 5 more minutes
<br>
Digested ligation with
:P only
:E/P
<br>
Ran on gel <br>
 
13.10.23_Pre_ext.jpg‎
 
<br>
Extracted the bottom bands from the two J06702 cut with E/X <br>
 
13.10.23_Post_ext.jpg‎
 
<br>
Zymo gel recover kit
<br>
{| {{table}}
|-
|plasmid||260||280||260/280||concentration
|-
|J06702 3||0.017 ||0.011 ||1.468|| 16.729
|-
|J06702 4||0.008 ||0.005|| 1.583||7.782
|-
|}




Revision as of 13:36, 24 October 2013

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mm/dd/yyyy

miniprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.

plasmid 260 280 260/280 concentration
J06702 0.247 0.128 1.93 247.2
ligation 0.071 0.035 2.006 70.9


Digested J06702 with

E only for 10 minutes
X only for 10 minutes
E and X for 10 minutes
E only for 5, then add X for 5 more minutes


Digested ligation with

P only
E/P


Ran on gel

13.10.23_Pre_ext.jpg‎


Extracted the bottom bands from the two J06702 cut with E/X

13.10.23_Post_ext.jpg‎


Zymo gel recover kit

plasmid 260 280 260/280 concentration
J06702 3 0.017 0.011 1.468 16.729
J06702 4 0.008 0.005 1.583 7.782



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