Haynes Lab:Notebook/Characterizing AHL quorum sensing homologs/2013/10/23: Difference between revisions
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Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
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Ran on gel <br> | Ran on gel <br> | ||
<br>Cut J06702 should be around 3kb. Possibly uncut, hence the crescent shapes and multiple bands.<br> | |||
[[image:13.10.23_Pre_ext.jpg |600px|pre-extraction]] | |||
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Extracted the bottom bands from the two J06702 cut with E/X <br> | Extracted the bottom bands from the two J06702 cut with E/X <br> | ||
Arrows show where the bands were extracted.<br> | |||
[[image:13.10.23_Post_ext.jpg |600px|post-extraction]] | |||
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Revision as of 13:40, 24 October 2013
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mm/dd/yyyyminiprepped J06702 and the ligation colony I picked (backbone control plate had as many colonies as the ligation). Good yield for J06702, ok for other.
Digested J06702 with
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